LOCUS       sequence-9874-ex        3751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.58
FEATURES             Location/Qualifiers
     source          1..3751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1796..1920
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1992..2011)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1993..2011)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2016..2032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2016..2032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2134..2153)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2145..2954
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2212..2231)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3101..3689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3590..3609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgac ctttaaataa aatcgagaaa aaatggcgtc
      721 aaaagacata tgtaaaagct gtttctcttt gtatattcac acagaatata gcccgatttt
      781 ggaggtgaaa aaccggtttt tttgtattct ttacgccaca taaagtgata tggaagagaa
      841 aatcgacata tttatgtact tggagtacga gtacattgga gaaaaagggt gaactactgg
      901 aaaatctggg aaatttcaga aaaaaattcc gaaaaatctt ttttgctgaa aaaaaaattt
      961 gttcgaaatt tgggaaatac tggaaaattt tcatcaaaat taaagcgatt tttaacgttt
     1021 ttgcttggga gatactattt gtaattttaa tgtttggtga ggacaacaga aatgaccttc
     1081 agcgtacgga aaacgattaa aagacacatt tgaatcgaaa atagcgtgaa aaacatgaaa
     1141 atatcggaaa aatagcttta aaattggatt tgaaagcaaa aaatgtatca aaaatcgaga
     1201 aaaatgcttg aaaaagttga aaaactcaaa caaaaaatca aaaaaggttt ttttaaaaat
     1261 ctgtgagtat cgcaacgaaa gcacaacttg aggcatgcgc ctttaaacca accgtaacca
     1321 aattcggagg caaaaaaaga tttctcgctg ttttttcagt tttaaaatca cttttcacgt
     1381 ttcttttcgc agtaattcgt ttgcaaaaca tctgaaaaaa ttttatttta taagaaatta
     1441 tataaaaatc gctataaatt gagtttttgc ccccaaattc agcacggagc acttctcaat
     1501 taaatataat tttatttttt aacattgaaa gctttcataa aattgagctt tgtttgtaaa
     1561 taatcagtga aaaaacacag aaatcttcaa aaaataaaga aaaagttttt aaaaatctgt
     1621 gagtcccgca acgaaaataa aactttaggc atgcgccttt aaaccaaccg taaccaaatt
     1681 cggcggcaaa aatggatttc tcgccgtttt ttcagtttta caatcacttt ttaggtttct
     1741 ttttgcagta tttcgtttcc caaacaatta aaaatcaaat tttcttttcc agatgacaag
     1801 tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     1861 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
     1921 aatcaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg
     1981 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     2041 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     2101 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2161 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2221 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2281 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2341 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2401 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2461 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2521 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2581 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2641 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2701 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2761 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2821 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2881 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2941 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3001 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3241 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3481 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3721 tggccttttg ctggcctttt gctcacatgt t
//
