LOCUS       sequence-98741-e         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP64 human MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP64 human MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             143..163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             173..322
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             329..355
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 taccatccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tcgagctcgg
       61 tacccgggga tcctctagtc agctgacgcg tttggttatg catgccacca tgaaaactag
      121 tggacaggcc ggccaggcta gcccgaaaaa gaaacgcaaa gttgggcgcg ccgacgcgct
      181 ggacgatttc gatctcgaca tgctgggttc tgatgccctc gatgactttg acctggatat
      241 gttgggaagc gacgcattgg atgactttga tctggacatg ctcggctccg atgctctgga
      301 cgatttcgat ctcgatatgt taattaacta cccgtacgac gttccggact acgcttctgg
      361 aggcagtggg ggcggatcaa tggagctact gtcgccaccg ctccgcgacg tagacctgac
      421 ggcccccgac ggctctctct gctcctttgc cacaacggac gacttctatg acgacccgtg
      481 tttcgactcc ccggacctgc gcttcttcga agacctggac ccgcgcctga tgc
//
