LOCUS       sequence-98744-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses p65 mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..208)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgcccttggt cacctgcagc ttggcggtct gggtgccctc gtagggcttg ccctcgccct
       61 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
      121 tgaagggctt gatgacgttc tcagtgctat ccatagggcc gggattctcc tccacgtcac
      181 cgcatgttag aagacttcct ctgccctcat gcataagcac ctgataaatc gcattggggt
      241 ttgagcctgc aggacactga ggggcggcgt cgggagacgg ggtctgggtt ccctgttctg
      301 tgtcgcttag ggatgccccc tctggcggac ccggaggcga ctctggtggt gcatctgcca
      361 aaagcagcgc aggcgcagcg gggctgtctg tggagatgcg ctccactatg ctggacaggc
      421 agtcgaggct cgacacagcc gcactcttcc ctggcctgga ctcgcgcacc gcctcactgt
      481 agtaggcggt gtcgtagcca ttctgccgcc gggggccgct tggggggccg ctgtaatcca
      541 tcatgccatc
//
