LOCUS       sequence-98777-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cyan fluorescent protein variant mTurquoise.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW 
            Jr
  TITLE     Bright cyan fluorescent protein variants identified by fluorescence 
            lifetime screening.
  JOURNAL   Nat Methods. 2010 Feb;7(2):137-9. doi: 10.1038/nmeth.1415. Epub 2010
            Jan 17.
  PUBMED    20081836
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1415"; journalName: "Nat Methods"; date: "2010-02"; 
            volume: "7"; issue: "2"; pages: "137-9"
COMMENT     Sequence Label: pmTurquoise-C1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(51..53,54..56,57..767)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(96..117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(357..376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     704..725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    768..833
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca
       61 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      241 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      481 acaactacat cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      541 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
      601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      661 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
      781 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
      841 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      901 acctcccaca c
//
