LOCUS       sequence-9878-ex        3749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.127
FEATURES             Location/Qualifiers
     source          1..3749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1795..1918
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1990..2009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1991..2009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2132..2151)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2143..2952
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2210..2229)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3099..3687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3588..3607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgac ctttaaataa aatcgagaaa aaatggcgtc
      721 aaaagacata tgtaaaagct gtttctcttt gtatattcac acagaatata gcccgatttt
      781 ggaggtgaaa aaccggtttt tttgtattct ttacgccaca taaagtgata tggaagagaa
      841 aatcgacata tttatgtact tggagtacga gtacattgga gaaaaagggt gaactactgg
      901 aaaatctggg aaatttcaga aaaaaattcc gaaaaatctt ttttgctgaa aaaaaaattt
      961 gttcgaaatt tgggaaatac tggaaaattt tcatcaaaat taaagcgatt tttaacgttt
     1021 ttgcttggga gatactattt gtaattttaa tgtttggtga ggacaacaga aatgaccttc
     1081 agcgtacgga aaacgattaa aagacacatt tgaatcgaaa atagcgtgaa aaacatgaaa
     1141 atatcggaaa aatagcttta aaattggatt tgaaagcaaa aaatgtatca aaaatcgaga
     1201 aaaatgcttg aaaaagttga aaaactcaaa caaaaaatca aaaaaggttt ttttaaaaat
     1261 ctgtgagtat cgcaacgaaa gcacaacttg aggcatgcgc ctttaaacca accgtaacca
     1321 aattcggagg caaaaaaaga tttctcgctg ttttttcagt tttaaaatca cttttcacgt
     1381 ttcttttcgc agtaattcgt ttgcaaaaca tctgaaaaaa ttttatttta taagaaatta
     1441 tataaaaatc gctataaatt gagtttttgc ccccaaattc agcacggagc acttctcaat
     1501 taaatataat tttatttttt aacattgaaa gctttcataa aattgagctt tgtttgtaaa
     1561 taatcagtga aaaaacacag aaatcttcaa aaaataaaga aaaagttttt aaaaatctgt
     1621 gagtcccgca acgaaaataa aactttaggc atgcgccttt aaaccaaccg taaccaaatt
     1681 cggcggcaaa aatggatttc tcgccgtttt ttcagtttta caatcacttt ttaggtttct
     1741 ttttgcagta tttcgtttcc caaacaatta aaaatcaaat tttcttttcc agatcaagtt
     1801 tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     1861 ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa
     1921 tcaactactt agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc
     1981 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2041 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     2101 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     2161 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     2221 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     2281 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2341 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     2401 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     2461 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2521 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2581 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2641 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2701 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2761 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2821 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2881 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2941 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3001 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     3061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3241 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3481 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3721 gccttttgct ggccttttgc tcacatgtt
//
