LOCUS       sequence-98780-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for expressing circular RNAs of a desired 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Liang D, Wilusz JE
  TITLE     Short intronic repeat sequences facilitate circular RNA production.
  JOURNAL   Genes Dev. 2014 Oct 3. pii: gad.251926.114.
  PUBMED    25281217
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2014 Oct 3. pii: gad.251926.114."
COMMENT     Sequence Label: pcDNA3.1(+) CircRNA Mini Vector
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(374..391)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    380..604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc aaagtgctga gattacaggc gtgagccacc acccccggcc cactttttgt
      181 aaaggtacgt actaatgact ttttttttat acttcaggat atcatcgata ccggtttaat
      241 taacacgtgg gtaaccgtta acccgcggag gtaagaagca aggaaaagaa ttaggctcgg
      301 cacggtagct cacacctgta atcccagcag cggccgctcg agtctagagg gcccgtttaa
      361 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
      601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcggg
//
