LOCUS       sequence-98790-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mNaChBac-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pCAG-mNaChBac-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    110..231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(147..166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     201..220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(299..317)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(338..354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(351..373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(386..392,393..410,411..416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    431..452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(569..586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
       61 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atttaaattg
      241 ggcgcgccca actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
      301 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      661 gggataacgc aggaaagaac atgtga
//
