LOCUS       sequence-98799-e        1547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and 
            Chitin-binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Picelli S, Bjorklund AK, Reinius B, Sagasser S, Winberg G, Sandberg 
            R
  TITLE     Tn5 transposase and tagmentation procedures for massively-scaled 
            sequencing projects.
  JOURNAL   Genome Res. 2014 Jul 30. pii: gr.177881.114.
  PUBMED    25079858
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Jul 30. pii: gr.177881.114."
COMMENT     Sequence Label: pTXB1-Tn5
FEATURES             Location/Qualifiers
     source          1..1547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..1477
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
ORIGIN
        1 gaattccctc tagaaataat tttgttaact ttaagaagga gatatacata tgattaccag
       61 tgcactgcat cgtgcggcgg attgggcgaa aagcgtgttt tctagtgctg cgctgggtga
      121 tccgcgtcgt accgcgcgtc tggtgaatgt tgcggcgcaa ctggccaaat atagcggcaa
      181 aagcattacc attagcagcg aaggcagcaa agccatgcag gaaggcgcgt atcgttttat
      241 tcgtaatccg aacgtgagcg cggaagcgat tcgtaaagcg ggtgccatgc agaccgtgaa
      301 actggcccag gaatttccgg aactgctggc aattgaagat accacctctc tgagctatcg
      361 tcatcaggtg gcggaagaac tgggcaaact gggtagcatt caggataaaa gccgtggttg
      421 gtgggtgcat agcgtgctgc tgctggaagc gaccaccttt cgtaccgtgg gcctgctgca
      481 tcaagaatgg tggatgcgtc cggatgatcc ggcggatgcg gatgaaaaag aaagcggcaa
      541 atggctggcc gctgctgcaa cttcgcgtct gagaatgggc agcatgatga gcaacgtgat
      601 tgcggtgtgc gatcgtgaag cggatattca tgcgtatctg caagataaac tggcccataa
      661 cgaacgtttt gtggtgcgta gcaaacatcc gcgtaaagat gtggaaagcg gcctgtatct
      721 gtatgatcac ctgaaaaacc agccggaact gggcggctat cagattagca ttccgcagaa
      781 aggcgtggtg gataaacgtg gcaaacgtaa aaaccgtccg gcgcgtaaag cgagcctgag
      841 cctgcgtagc ggccgtatta ccctgaaaca gggcaacatt accctgaacg cggtgctggc
      901 cgaagaaatt aatccgccga aaggcgaaac cccgctgaaa tggctgctgc tgaccagcga
      961 gccggtggaa agtctggccc aagcgctgcg tgtgattgat atttataccc atcgttggcg
     1021 cattgaagaa tttcacaaag cgtggaaaac gggtgcgggt gcggaacgtc agcgtatgga
     1081 agaaccggat aacctggaac gtatggtgag cattctgagc tttgtggcgg tgcgtctgct
     1141 gcaactgcgt gaatctttta ctccgccgca agcactgcgt gcgcagggcc tgctgaaaga
     1201 agcggaacac gttgaaagcc agagcgcgga aaccgtgctg accccggatg aatgccaact
     1261 gctgggctat ctggataaag gcaaacgcaa acgcaaagaa aaagcgggca gcctgcaatg
     1321 ggcgtatatg gcgattgcgc gtctgggcgg ctttatggat agcaaacgta ccggcattgc
     1381 gagctggggt gcgctgtggg aaggttggga agcgctgcaa agcaaactgg atggctttct
     1441 ggccgcgaaa gacctgatgg cgcagggcat taaaatctgc atcacgggag atgcactagt
     1501 tgccctaccc gagggcgagt cggtacgcat cgccgacatc gtgccgg
//
