LOCUS       sequence-9880-ex        3505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.151
FEATURES             Location/Qualifiers
     source          1..3505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1681..1780)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1892..1911)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1903..2712
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1970..1989)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2855..3443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3344..3363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      421 aaagcaggct caaaaatggt ctccaaggga gaggaggata acatggctat catcaaggag
      481 ttcatgagat tcaaggtcca catggaggga tccgtcaacg gacacgagtt cgagatcgag
      541 ggagagggag agggaagacc atacgaggga acccagaccg ctaagcttaa ggtcaccaag
      601 ggaggaccag taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
      661 cttccattcg cttgggatat cctttcccca cagttcatgt acggatccaa ggcttacgtc
      721 aagcacccag ctgatatccc agattacctt aagctttcct tcccagaggg attcaagtgg
      781 gagagagtca tgaacttcga ggatggagga gtcgtcaccg tcacccagga ttcctccgta
      841 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagct tcaggatgga
      901 gagttcatct acaaggtcaa gcttagagga accaacttcc catccgatgg accagtcatg
      961 cagaagaaga ccatgggatg ggaggcttcc tccgagagaa tgtacccaga ggatggagct
     1021 cttaagggag agatcaagca gagacttaag cttaaggatg gagtaagttt aaacatgatt
     1081 ttactaacta actaatctga tttaaatttt cagggacact acgatgctga ggtcaagacc
     1141 acctacaagg ctaagaagcc agtccagctt ccaggagctt acaacgtcaa catcaagctt
     1201 gatatcacct cccacaacga ggattacacc atcgtcgagc agtacgagag agctgaggga
     1261 agacactcca ccggaggaat ggatgagctt tacaagggat caggtagtgg cccaccaaag
     1321 ccatctgcca agggagccaa gaaggccgcc aagaccgtcg ttgccaagcc aaaggacgga
     1381 aagaagagac gtcatgcccg caaggaatcg tactccgtct acatctaccg tgttctcaag
     1441 caagttcacc cagacaccgg agtctcctcc aaggccatgt ctatcatgaa ctccttcgtc
     1501 aacgatgtat tcgaacgcat cgcttcggaa gcttcccgtc ttgctcatta caacaaacgc
     1561 tcaacgatct catcccgcga aattcaaacc gctgtccgtt tgattctccc aggagaactt
     1621 gccaagcacg ccgtgtctga gggaaccaag gccgtcacca agtacacttc cagcaagtaa
     1681 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1741 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
     1801 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1861 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1921 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1981 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     2041 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2101 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     2161 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     2221 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2281 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2341 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2401 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2461 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2521 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2581 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2641 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2701 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2761 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3001 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3481 tttgctggcc ttttgctcac atgtt
//
