LOCUS       sequence-98805-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and 
            Chitin-binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Picelli S, Bjorklund AK, Reinius B, Sagasser S, Winberg G, Sandberg 
            R
  TITLE     Tn5 transposase and tagmentation procedures for massively-scaled 
            sequencing projects.
  JOURNAL   Genome Res. 2014 Jul 30. pii: gr.177881.114.
  PUBMED    25079858
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Jul 30. pii: gr.177881.114."
COMMENT     Sequence Label: pTXB1-Tn5
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(83..130)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     126..144
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(209..364)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtagttatc
       61 cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg
      121 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
      181 tagcagccgg atccccttcc tgcagtcatt gaagctgcca caaggcagga acgttggatg
      241 gttcccatcc tgccaaggag gtgtggggct gcaaacattt atacgtcttg ccgttatatg
      301 tgaccaattg tcccgcagta taagctgtgt tgacctgcca agcggataca ccaggatttg
      361 tcgtgaggcc tgagttcaga ccggtgaggc cagtagcgtg gctgacgaac ccgttcgtga
      421 taaacgcgtg gtctgccgtg tcgacacgaa ggctatacac cggctgcacg ccggcgtcgg
      481 tgacactggc gactttcgcg tagtagaacc gcccgtcggt cagctcgtcg gcgatagctt
      541 gggcgtccgg gtctcggtgg tgtgcttcca agaaacgcac cagtccaggg acgccgactg
      601 tgtaggttgt gggcgcaaat tcgggtttcc cgcgggcaaa acctgcacag tcgacgctga
      661 atgcgctgcg ttgaatcacc gcgtaatcgc ccggcttgat
//
