LOCUS       sequence-98818-e         227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses pCAG-Flpo; codon-optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pCAG-Flpo
FEATURES             Location/Qualifiers
     source          1..227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..62
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attctgcagt cgacggtacc ggatccgccg ccaccatggc tcctaagaag aagaggaagg
       61 tgatgagcca gttcgacatc ctgtgcaaga ccccccccaa ggtgctggtg cggcagttcg
      121 tggagagatt cgagaggccc agcggcgaga agatcgccag ctgtgccgcc gagctgacct
      181 acctgtgctg gatgatcacc cacaacggca ccgccatcaa gagggcc
//
