LOCUS       sequence-9882-ex        5644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.157
FEATURES             Location/Qualifiers
     source          1..5644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1714..1742)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2490..2510
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(3753..3848)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3885..3904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3886..3904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3909..3925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3909..3925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4027..4046)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4038..4847
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4105..4124)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4994..5582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5483..5502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggat aaaaagttca catttataac tcacactccc
      781 tttgttttgt tcgttctcac cattttaggt taccatagaa tcatgtgcca tacatcatgt
      841 tgccattttt tccccctctc atctccccca tctttatcat gtcatcaatt ggtatctacc
      901 atctcactcc tttaggaata gcatacacat acagttagta ataattatgt aatgagatac
      961 ctaataccat taattccttt ctgtaattta aactcgccct gctgctttca ccatatgctc
     1021 tttgattttt aagtatcttt tccctttctt cgcagatctt gaaaagatat tttaacatca
     1081 gagaaaaacg gcaggtgccc cggtaataac atcttatcag gataaccatt ttggagaata
     1141 ctcattgata cttttattga tgcagctatt cccctaccca ttcatactac ctcgaatgcc
     1201 aaagcacccc ctttattttt ctgtgttatc ttaacgctaa cccctgtaga atcttcccgg
     1261 tttgatgtag tcatcaaaaa tttttcatct tttctaaatt tttagtaact tgagatttta
     1321 attttctttc ccgctagtta gatatgttca aatctcacat ccccatcaaa ccagccccaa
     1381 gtccccattc taaaccccta ccccttccca atctgtccat tataatttta agcacaagtc
     1441 caagagctaa tatttttagc agtctttcac attgaccatt ttctcggtac cctgtttttg
     1501 agaccaaaat tttgaatttt gaaattgaaa tacagggcga gataaaataa taaaggttgt
     1561 atatttattc atcttattga atctgccatt tcctcgtttt tgcgagttta tataccttcc
     1621 aattttcttt ctattgtatt ttcaacttct aattttaatt cagggaaact gcttcaacgc
     1681 atcatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1741 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1801 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atgggtaagt
     1861 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact
     1921 actttctgtt atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac
     1981 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     2041 gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc atacatattt
     2101 aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     2161 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     2221 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttgtaagt
     2281 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt caaaattaga
     2341 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     2401 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     2461 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     2521 attacacatg gcatggatga actatacaaa tgcccggggg atcggtggag ctccaccggt
     2581 ggcggccgct ctagaactag tccaccaaag ccatctgcca agggagccaa gaaggccgcc
     2641 aagaccgtcg ttgccaagcc aaaggacgga aagaagagac gtcatgcccg caaggaatcg
     2701 tactccgtct acatctaccg tgttctcaag caagttcacc cagacaccgg agtctcctcc
     2761 aaggccatgt ctatcatgaa ctccttcgtc aacgatgtat tcgaacgcat cgcttcggaa
     2821 gcttcccgtc ttgctcatta caacaaacgc tcaacgatct catcccgcga aattcaaacc
     2881 gctgtccgtt tgattctccc aggagaactt gccaagcacg ccgtgtctga gggaaccaag
     2941 gccgtcacca agtacacttc cagcaagtaa aataacccgc aataggattc ttttatcatc
     3001 gaaatttctg tagattactt acttcaaata atcatgcaca caggttgtcc ccgttaattc
     3061 tttccttcaa tctctttctc tctttcacct agtctgtgcc atttcaatat gattgcaccc
     3121 cggaaaatat caaaaacttc acttaacaag tgatcccata ttacaaaaat attcaaattc
     3181 cgaaaatcga aagatgcatt tccgttttat tttaccgaat tttgatgttt ttgtaatgtg
     3241 tttaaacccc ttttacaatt aacttattaa tgttttgttt tactcatctc acttcccctt
     3301 cttactttgt gcttatttta tgttttaggt ttttttattg tatcataaaa ctggaaattt
     3361 tgaaataggc ttctttctcc ctaaactttt ttaacaggta ctgtaactgg tacatatcct
     3421 ctgccccacc ccgctcccaa ctcgtctttg tctgtttgtt tatacgaaat acaattttta
     3481 acgagtatgc gggtttctgt tagcttcaac aatacgatca ttcaaatttt aaaattaaga
     3541 aactataaaa gcacagtatt tacgtgagtt gaaacaaaat gccttctcca aatttcttaa
     3601 cactgaaaaa ttttcggaaa aaaagcagaa attactccgc aaaaaattaa ggacatcatt
     3661 ccaatatttt tttttactta tttgatgaaa aattaatata ttccaataaa ttaaaatgaa
     3721 ttatcatctc aggaatactg tagaattact ttcaacttta ttatacaaag ttggcattat
     3781 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3841 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt
     3901 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3961 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     4021 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     4081 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     4141 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     4201 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     4261 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     4321 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     4381 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     4441 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     4501 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     4561 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4621 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4681 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4741 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4801 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     4861 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     4921 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5641 tgtt
//
