LOCUS       sequence-98824-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCMrfp with TrfA mutation (271R to C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Bi C, Su P, Muller J, Yeh YC, Chhabra SR, Beller HR, Singer SW, 
            Hillson NJ
  TITLE     Development of a broad-host synthetic biology toolbox for Ralstonia 
            eutropha and its application to engineering hydrocarbon biofuel 
            production.
  JOURNAL   Microb Cell Fact. 2013 Nov 13;12:107. doi: 10.1186/1475-2859-12-107.
  PUBMED    24219429
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-11-13"; volume: "12:107. doi"; pages: " 
            10.1186/1475-2859-12-107"
COMMENT     Sequence Label: pCM271rfp
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..757
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTEHMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt ttgggaattc aaaagatctt
       61 ttaagaagga gatatacata tggcgagtag cgaagacgtt atcaaagagt tcatgcgttt
      121 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      181 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      241 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      301 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      361 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      421 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      481 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacatatgt acccggaaga
      541 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      601 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      661 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      721 cgaacgtgct gaaggtcgtc actccaccgg tgcttaagga tccaaactcg agtaaggatc
      781 tccaggcatc aaataaaacg aa
//
