LOCUS       sequence-9883-ex        4862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.23
FEATURES             Location/Qualifiers
     source          1..4862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3038..3137)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3249..3268)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3260..4069
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3327..3346)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4212..4800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4701..4720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct taatgccgtc gtgcaccact ccaacttacg gtgtttcgac ccaactagaa
      481 agccaaagct ctgaatcgcc atctcgttcg tccgttatga cgccaacgtc tctggatgga
      541 gacaatagtc cacggaagag attccccatc atcgacaatg ttccagcgga tcgttggccg
      601 tcaactcggc gagacggatg ggtacgcgaa ttttcatttt gttcatcaat tacagtaata
      661 aaaaattttc agagctctgt tagagcgcca ccaccagctc gtctgacgct atcgacgaac
      721 aacagacaca tcatgtcccc gatttcgagc gcttacagtc aaacacctaa ttcgcttttg
      781 tcgccagcca tgttcaatcc aaagagcaga agtatctttt ccccgactct tcctgcaact
      841 ccaatgagtt atggaaaatc atcgatggac aagagtatga aacaatctca tgtctactta
      901 tttttctgta atttcttcag gcctgttctc tccaacagct acagaaccaa tcgaggttga
      961 ggcaactgtc gagtacttgg ctgatctggt taaagagaag aaacatctta cgctctttcc
     1021 tcatatgttc agcaatgttg aacgtttgtt ggatgatggt acataaccgc tctgtgttcc
     1081 tacaagtaat tattttattt tagaaatcgg tcgtgtccgc gtggctttgt tccaaactga
     1141 gtttccgaga gttgagcttc ctgagcctgc tggagacatg atttcgatca ctgagaagat
     1201 ttatgttcca aagaacgaat acccagacgt aatttaacga atttcaagtt ttcgaacaac
     1261 taaaacattt cagtataact tcgtcggtag aattctcggc ccacgtggga tgaccgccaa
     1321 gcagcttgag caggacactg gatgcaagat tatggtccga ggaaagggat caatgcggga
     1381 caagtcaaag gtttgatctt gttacaattt tatctaaaac aaagtttagt tgttgaagga
     1441 aagtgctcac cgtggaaagg ccaattggga gcatctcgaa gacgatctgc acgttcttgt
     1501 gcaatgcgaa gacactgaga atcgtgttca catcaaattg caagcagcac tcgaacaggt
     1561 caagaagttg ctcattccgg ctgtgagtta tatgcttcgt aaaatctcta caataaccta
     1621 ttttcagccc gagggaactg acgagctgaa gagaaaacaa cttatggaac ttgcaatcat
     1681 caacggtacg tatcgtccaa tgaagtcgcc aaacccagct cgtgtgatga ctgcagttcc
     1741 tctgctctct ccaactccac tacgtagttc aggtccagtt ttgatgtcgc caactcctgg
     1801 atcaggactc ccgtcaacga catttggagg atcgatcttg gtagaacagt tcttcagttt
     1861 aagtttcata tttttaattt cagagcccca ctctcactgc atccaacctt ctcggatcta
     1921 acgtcttcga ttactcgctt ctgagcccga gcatgtttga ttcattcagc tctcttcaac
     1981 ttgcaagtga tttgacgttc ccgaagtacc caacaaccac ttcgtttgtc aactcattcc
     2041 ctggtctttt cacttctgca tcttcttttg ccaatcaaac aaacaccaat gtgtccccga
     2101 gtggagcaag tccctcggct tcttcagtca acaacacctc tttctaaaaa gttcacattt
     2161 ataactcaca ctccctttgt tttgttcgtt ctcaccattt taggttacca tagaatcatg
     2221 tgccatacat catgttgcca ttttttcccc ctctcatctc ccccatcttt atcatgtcat
     2281 caattggtat ctaccatctc actcctttag gaatagcata cacatacagt tagtaataat
     2341 tatgtaatga gatacctaat accattaatt cctttctgta atttaaactc gccctgctgc
     2401 tttcaccata tgctctttga tttttaagta tcttttccct ttcttcgcag atcttgaaaa
     2461 gatattttaa catcagagaa aaacggcagg tgccccggta ataacatctt atcaggataa
     2521 ccattttgga gaatactcat tgatactttt attgatgcag ctattcccct acccattcat
     2581 actacctcga atgccaaagc acccccttta tttttctgtg ttatcttaac gctaacccct
     2641 gtagaatctt cccggtttga tgtagtcatc aaaaattttt catcttttct aaatttttag
     2701 taacttgaga ttttaatttt ctttcccgct agttagatat gttcaaatct cacatcccca
     2761 tcaaaccagc cccaagtccc cattctaaac ccctacccct tcccaatctg tccattataa
     2821 ttttaagcac aagtccaaga gctaatattt ttagcagtct ttcacattga ccattttctc
     2881 ggtaccctgt ttttgagacc aaaattttga attttgaaat tgaaatacag ggcgagataa
     2941 aataataaaa aatagttttt attcaatttt tcttccagtt catgttttaa aacgattaaa
     3001 aaggaaacat tagttagaaa cattttcatt tcttcaaacc cagctttctt gtacaaagtg
     3061 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     3121 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
     3181 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3241 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3301 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3361 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3421 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3481 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3541 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3601 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3661 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3721 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3781 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3841 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3901 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3961 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     4021 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     4081 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     4141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4861 tt
//
