LOCUS       sequence-98836-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for expressing circular RNAs of a desired 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Liang D, Wilusz JE
  TITLE     Short intronic repeat sequences facilitate circular RNA production.
  JOURNAL   Genes Dev. 2014 Oct 3. pii: gad.251926.114.
  PUBMED    25281217
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2014 Oct 3. pii: gad.251926.114."
COMMENT     Sequence Label: pcDNA3.1(+) CircRNA Mini Vector
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(294..313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(295..313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(358..561)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(387..407)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        562..865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tctagactcg agcggccgct gctgggatta caggtgtgag ctaccgtgcc gagcctaatt
       61 cttttccttg cttcttacct ccgcgggtta acggttaccc acgtgttaat taaaccggta
      121 tcgatgatat cctgaagtat aaaaaaaaag tcattagtac gtacctttac aaaaagtggg
      181 ccgggggtgg tggctcacgc ctgtaatctc agcactttga attccaccac actggactag
      241 tggatccgag ctcggtacca agcttaagtt taaacgctag ccagcttggg tctccctata
      301 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      361 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      421 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      481 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      541 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      601 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      661 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      721 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      781 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      841 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
//
