LOCUS       sequence-98837-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Nan X, Collisson EA, Lewis S, Huang J, Tamguney TM, Liphardt JT, 
            McCormick F, Gray JW, Chu S
  TITLE     Single-molecule superresolution imaging allows quantitative analysis
            of RAF multimer formation and signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18519-24. doi: 
            10.1073/pnas.1318188110. Epub 2013 Oct 24.
  PUBMED    24158481
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1318188110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-11-12- 12"; volume: "110"; issue: "46"; pages: 
            "18519-24"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1-MCS
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    64..163
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(359..377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     627..646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
       61 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      121 caatgctttt ttataatgcc aactttgtac aaaaaagcag gctccgcagc cgcccccttc
      181 accaagcttc tcgagctgca ggagctcggt accatggtga gcaagggcga ggaggataac
      241 atggccatca tcaaggagtt catgcgcttc aaggtgcata tggagggctc cgtgaacggc
      301 cacgtgttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      361 aagctgaagg tgaccaaggg tggccccctg cccttcacct gggacatcct gtcccctcag
      421 ttcatgtacg gctccaacgc ctacgtgaag caccccgccg acatccccga ctacttcaag
      481 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga agttcgagga cggcggcgtg
      541 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      601 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      661 gccctctccg agcggatgta ccccgaggac ggcgccctga agggcgaggt caagccaagg
      721 gtcaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      781 aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca agttggacat cacctcccac
      841 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      901 ggcatg
//
