LOCUS       sequence-98838-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding iCas9, tracrRNA and crRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
  TITLE     Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step 
            Multigene Disruption in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2014 Sep 19.
  PUBMED    25207793
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Sep 19."
COMMENT     Sequence Label: pCRCT
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..77
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             81..101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttcttgctc attagaaaga aagcatagca atctaatcta agttttctag atggattata
       61 aagatgacga tgacaaacct ccaaaaaaga agagaaaggt cgataagaaa tactcaatag
      121 gcttagatat cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc
      181 cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta
      241 taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag
      301 ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa
      361 atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg
      421 aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt
      481 atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat tctacttata
      541 aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt
      601 ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt
      661 tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg
      721 ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaa
//
