LOCUS       sequence-98842-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Nickerson A, Huang T, Lin LJ, Nan X
  TITLE     Photoactivated localization microscopy with bimolecular fluorescence
            complementation (BiFC-PALM) for nanoscale imaging of protein-protein
            interactions in cells.
  JOURNAL   PLoS One. 2014 Jun 25;9(6):e100589. doi: 
            10.1371/journal.pone.0100589. eCollection 2014.
  PUBMED    24963703
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-25"; volume: "9(6):e100589. doi"; pages: " 
            10.1371/journal.pone.0100589. eCollection 2014"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1_1-159-MCS
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    61..160
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(356..374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     624..643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(707..806)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
       61 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
      121 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcagccgc ccccttcacc
      181 aagcttctcg agctgcagga gctcggtacc atggtgagca agggcgagga ggataacatg
      241 gccatcatca aggagttcat gcgcttcaag gtgcatatgg agggctccgt gaacggccac
      301 gtgttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      361 ctgaaggtga ccaagggtgg ccccctgccc ttcacctggg acatcctgtc ccctcagttc
      421 atgtacggct ccaacgccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg
      481 tccttccccg agggcttcaa gtgggagcgc gtgatgaagt tcgaggacgg cggcgtggtg
      541 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      601 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      661 ctctccgagc ggatgtaccc cgaggactaa aagggtgggc gcgccgaccc agctttcttg
      721 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      781 atcagtcaaa ataaaatcat tatttgcc
//
