LOCUS       sequence-98844-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses His-tagged B18R protein from Western vaccinia virus that 
            binds to and neutralizes type I IFNs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: pTNT-B18R-6His
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      306..353
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(535..554)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgtcattat tgattgacga tgtactgatt gaatgggaaa atccatccgg atggcttata
       61 ggattcgatt ttgatgtata ctctgtttta actagtagag gcggtattac cgaggcgacc
      121 ttgtactttg aaaatgttac tgaagaatat ataggtaata catataaatg tcgtggacac
      181 aactattatt ttgaaaaaac ccttacaact acagtagtat tggagaagct tgcggccggt
      241 ggtggccatc atcatcatca tcactgagcg gccgcaaaaa aaaaaaaaaa aaaaaaaaaa
      301 aaaaactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttggatcccg
      361 gcgcgccggg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      421 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
      481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      661 attagggtga
//
