LOCUS       sequence-98846-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding iCas9 and tracrRNA on pRS415 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
  TITLE     Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step 
            Multigene Disruption in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2014 Sep 19.
  PUBMED    25207793
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Sep 19."
COMMENT     Sequence Label: pCT
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcaatctaa tctaagtttt ctagatggat tataaagatg acgatgacaa acctccaaaa
       61 aagaagagaa aggtcgataa gaaatactca ataggcttag atatcggcac aaatagcgtc
      121 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
      181 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
      241 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
      301 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
      361 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
      421 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
      481 catctgcgaa aaaaattggt agattctact tataaagcgg atttgcgctt aatctatttg
      541 gccttagcgc atatgattaa gtttcgtggt cattttttga ttg
//
