LOCUS       sequence-9886-ex        3426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.54
FEATURES             Location/Qualifiers
     source          1..3426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1535..1630)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1667..1686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1668..1686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1691..1707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1691..1707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1809..1828)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1820..2629
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1887..1906)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2776..3364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3265..3284
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggat aaaataaccc gcaataggat tcttttatca
      781 tcgaaatttc tgtagattac ttacttcaaa taatcatgca cacaggttgt ccccgttaat
      841 tctttccttc aatctctttc tctctttcac ctagtctgtg ccatttcaat atgattgcac
      901 cccggaaaat atcaaaaact tcacttaaca agtgatccca tattacaaaa atattcaaat
      961 tccgaaaatc gaaagatgca tttccgtttt attttaccga attttgatgt ttttgtaatg
     1021 tgtttaaacc ccttttacaa ttaacttatt aatgttttgt tttactcatc tcacttcccc
     1081 ttcttacttt gtgcttattt tatgttttag gtttttttat tgtatcataa aactggaaat
     1141 tttgaaatag gcttctttct ccctaaactt ttttaacagg tactgtaact ggtacatatc
     1201 ctctgcccca ccccgctccc aactcgtctt tgtctgtttg tttatacgaa atacaatttt
     1261 taacgagtat gcgggtttct gttagcttca acaatacgat cattcaaatt ttaaaattaa
     1321 gaaactataa aagcacagta tttacgtgag ttgaaacaaa atgccttctc caaatttctt
     1381 aacactgaaa aattttcgga aaaaaagcag aaattactcc gcaaaaaatt aaggacatca
     1441 ttccaatatt tttttttact tatttgatga aaaattaata tattccaata aattaaaatg
     1501 aattatcatc tcaggaatac tgtagaatta ctttcaactt tattatacaa agttggcatt
     1561 ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1621 atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct atagtgagtc
     1681 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1741 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     1801 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     1861 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1921 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1981 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2041 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2101 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2161 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2221 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2281 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2341 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2401 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     2461 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     2521 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     2581 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     2641 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     2701 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2761 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2821 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2881 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2941 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3001 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3061 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     3181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3421 catgtt
//
