LOCUS       sequence-98867-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  crRNA expression plasmid on pRS423 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
  TITLE     Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step 
            Multigene Disruption in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2014 Sep 19.
  PUBMED    25207793
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Sep 19."
COMMENT     Sequence Label: pCR
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(183..370)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(517..536)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaccctatac ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct
       61 tctgcctctt tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag
      121 atcgcaatct gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc
      181 atctttgcct tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca
      241 ttactttata taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga
      301 gtcatccgct aggtggaaaa aaaaaaatga aaatcattac cgaggcataa aaaaatatag
      361 agtgtactag aggaggccaa gagtaataga aaaagaaaat tgcgggaaag gactgtgtta
      421 tgacttccct gactaatgcc gtgttcaaac gatacctggc agtgactcct agcgctcacc
      481 aagctcttaa aacgggaatt tatggtgcac tctcagtaca atctgctctg atgccgcata
      541 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      601 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtc
//
