LOCUS       sequence-98877-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, 
            Marks DL, Pagano RE
  TITLE     Rab proteins mediate Golgi transport of caveola-internalized 
            glycosphingolipids and correct lipid trafficking in Niemann-Pick C 
            cells.
  JOURNAL   J Clin Invest. 2002 Jun . 109(12):1541-50.
  PUBMED    12070301
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2002 Jun . 109(12):1541-50."
COMMENT     Sequence Label: DsRed-rab11 WT
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(61..63,64..66,67..738)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(244..264)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     462..481
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     656..679
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    742..798
                     /label=MCS
                     /note="multiple cloning site"
     CDS             802..828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             829..855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atggtgcgct cctccaagaa cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
      121 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      181 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      241 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
      301 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      361 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
      421 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
      481 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      541 gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
      601 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg
      661 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
      721 cgccaccacc tgttcctgct cagatctcga gctcaagctt cgaattctgc agtcgacggt
      781 accgcgggcc cgggatccat gtacccatac gatgttccgg attacgctta cccatacgat
      841 gttccggatt acgctggtac ccgcgacgac gagtacgact acctctttaa agttgtcctt
      901 attggagatt ctggtgttgg aaagagtaat ctcctgtctc ga
//
