LOCUS       sequence-98879-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of the fluorescent voltage sensor ASAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   St-Pierre F, Marshall JD, Yang Y, Gong Y, Schnitzer MJ, Lin MZ
  TITLE     High-fidelity optical reporting of neuronal electrical activity with
            an ultrafast fluorescent voltage sensor.
  JOURNAL   Nat Neurosci. 2014 Apr 22. doi: 10.1038/nn.3709.
  PUBMED    24755780
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2014 Apr 22. doi: 10.1038/nn.3709."
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-ASAP1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcctcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
       61 ccaatgccct ggctcacaaa taccactgag atctgcatga gctagccgcc accatggaga
      121 cgactgtgag gtatgaacag gggtcagagc tcactaaaac ttcgagctct ccaacagcag
      181 atgagcccac gataaagatt gatgatggtc gtgatgaggg taatgaacaa gacagctgtt
      241 ccaataccat taggagaaaa atttccccgt ttgtgatgtc atttggattc agagtatttg
      301 gagttgtgct tatcattgta gacatcatag tggtgattgt ggatctggcc atcagtgaga
      361 agaaaagagg cattagagag attcttgaag gtgtttccct ggctatagca ctcttcttcc
      421 ttgttgatgt tctcatgaga gtgtttgttg aaggcttcaa gaactatttc cggtccaaac
      481 tgaatacttt ggatgcagtc atagtagtgg gcactctgct aattaatatg acctactcct
      541 tctctgacct tgctgccttt aacagccata acgtgtatat taccgcggat aaacagaaaa
      601 acggcattaa agcgaacttt accgtgcgcc ataacgtgga agatggcagc gtgcagctgg
      661 cggatcatta tcagcagaac accccgattg gcgatggccc ggtgctgctg ccggataacc
      721 attatctgag cacccagacc gtgctgagca aagatccgaa cgaaaaacgc gatcacatgg
      781 tgctgctgga atttgtgacc gcagcgggca ttacacacgg catggatgaa ctgtatggcg
      841 gcaccggcgg cagcgcgagc cagggcgaag aactgtttac cggcgtggtg ccgattctgg
      901 tggaactgga tggcgatgtg aacggccata aatttagcgt gcgcggcgaa ggcgaaggcg
      961 atgcgaccat tggcaaactg accctgaaat ttatttgc
//
