LOCUS       sequence-98886-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Kir2.1Mut-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pCAG-Kir2.1Mut-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    109..230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(146..165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     200..219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(298..316)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(337..353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(337..353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(350..372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    361..377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(385..391,392..409,410..415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    430..451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(568..585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
       61 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
      121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
      181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tttaaattgg
      241 gcgcgcccaa ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
      301 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      541 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      661 ggataacgca ggaaagaaca tgtgagcaaa aggccagc
//
