LOCUS       sequence-98888-e         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the RBD of mouse MSI1 in bacterial cells for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctttaagaag gagatataca tatgcatcat catcatcatc acggcgaaaa cctgtatttt
       61 cagggcggat cccagcccgg cctcgcctcc ccggactcgc cgcacgaccc ctgcaagatg
      121 ttcatcggag gactcagttg gcagaccacg caggaagggc tgcgcgaata cttcggccag
      181 ttcggggagg tgaaagagtg tctggtgatg cgggaccccc tgaccaaaag atccaggggt
      241 ttcggcttcg tcactttcat ggaccaggcg ggggtggata aagtgctggc gcaatcgcgg
      301 cacgagctcg actccaaaac aattgacccc aaggtggcct ttcctcgaag agcacagcct
      361 aagatggtca ctcggacgaa gaagatcttc gtgggggggc tgtctgtgaa caccacggtg
      421 gaagatgtga aacactattt cgagcagttc ggaaaggtgg atgatgccat gctgatgttc
      481 gacaaaacca ccaacaggca cagagggttt ggatttgtca cgtttgagag cgaggacatc
      541 gta
//
