LOCUS       sequence-98892-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G35E mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)G35E
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg catcatcatc atcatcacgg cgaaaacctg
       61 tattttcagg gcggatccca gcccggcctc gcctccccgg actcgccgca cgacccctgc
      121 aagatgttca tcggaggact cagttggcag accacgcagg aagaactgcg cgaatacttc
      181 ggccagttcg gggaggtgaa agagtgtctg gtgatgcggg accccctgac caaaagatcc
      241 aggggtttcg gcttcgtcac tttcatggac caggcggggg tggataaagt gctggcgcaa
      301 tcgcggcacg agctcgactc caaaacaatt gaccccaagg tggcctttcc tcgaagagca
      361 cagcctaaga tggtcactcg gacgaagaag atcttcgtgg gggggctgtc tgtgaacacc
      421 acggtggaag atgtgaaaca ctatttcgag cagttcggaa aggtggatga tgccatgctg
      481 atgttcgaca aaaccaccaa caggcacaga gggtttggat ttgtcacgtt tgagagcgag
      541 gacatcgtag agaaagtttg tgagatccac ttccatgaaa tcaacaacaa aatggtggaa
      601 tgcaagaaag cccagccaaa ggaggtgatg tccccgtgaa agcttgcggc cgcactcgag
//
