LOCUS       sequence-98898-e         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R53E mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)R53E
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcatcatc atcatcatca cggcgaaaac
       61 ctgtattttc agggcggatc ccagcccggc ctcgcctccc cggactcgcc gcacgacccc
      121 tgcaagatgt tcatcggagg actcagttgg cagaccacgc aggaagggct gcgcgaatac
      181 ttcggccagt tcggggaggt gaaagagtgt ctggtgatgg aagaccccct gaccaaaaga
      241 tccaggggtt tcggcttcgt cactttcatg gaccaggcgg gggtggataa agtgctggcg
      301 caatcgcggc acgagctcga ctccaaaaca attgacccca aggtggcctt tcctcgaaga
      361 gcacagccta agatggtcac tcggacgaag aagatcttcg tgggggggct gtctgtgaac
      421 accacggtgg aagatgtgaa acactatttc gagcagttcg gaaaggtgga tgatgccatg
      481 ctgatgttcg acaaaaccac caacaggcac agagggtttg gatttgtcac gtttgagagc
      541 gaggacatcg tagagaaagt ttgtgagatc cacttccatg aaatcaacaa caaaatggtg
      601 gaatgcaaga aagcccagcc aaagga
//
