LOCUS       sequence-98902-e         537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP 
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: gRNA-eGFP-Reporter
FEATURES             Location/Qualifiers
     source          1..537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     188..210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     202..219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     203..219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     229..248
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        229..247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        262..510
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     262..282
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
       61 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      121 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      181 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
      241 ctatagggcg aattgggtac cgagggccta tttcccatga ttccttcata tttgcatata
      301 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
      361 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      421 tgttttaaaa tggactatca tatgcttatc gtaacttgaa agtatttcga tttcttggct
      481 ttatatatct tgtggaaagg acgaaacacc gaaaggtcga gaaactgcaa agtttta
//
