LOCUS       sequence-98907-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R53E R59E R61E triple mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)R53E/R59E/R61E
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             663..680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgtttaact ttaagaagga gatatacata tgcatcatca tcatcatcac ggcgaaaacc
       61 tgtattttca gggcggatcc cagcccggcc tcgcctcccc ggactcgccg cacgacccct
      121 gcaagatgtt catcggagga ctcagttggc agaccacgca ggaagggctg cgcgaatact
      181 tcggccagtt cggggaggtg aaagagtgtc tggtgatgga ggaccccctg accaaagagt
      241 ccgagggttt cggcttcgtc actttcatgg accaggcggg ggtggataaa gtgctggcgc
      301 aatctcggca cgagctcgac tccaaaacaa ttgaccccaa ggtggccttt cctcggagag
      361 cacagcctaa gatggtcact cgaactaaga agatctttgt gggggggctg tctgtgaaca
      421 ccacggtgga ggacgtgaag cactattttg agcagttcgg aaaggtggat gatgccatgc
      481 tgatgttcga caaaaccacc aacaggcaca gagggttcgg gtttgtcacg ttcgagagtg
      541 aggacatcgt agagaaagtg tgtgagatcc acttccatga aatcaacaac aaaatggtgg
      601 aatgcaagaa agctcagcca aaggaggtga tgtccccgtg aaagcttgcg gccgcactcg
      661 agcaccacca ccaccaccac tgagatccgg c
//
