LOCUS       sequence-98909-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eGFP reporter containing 8 copies of a gRNA binding site for 
            light-inducible dCas9 activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pGL3-Basic-8x-gRNA-eGFP
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    251..299
                     /note="synthetic polyadenylation signal"
     misc_feature    313..404
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     353..372
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        611..649
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      121 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      181 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
      241 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      301 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      361 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag
      421 ctcttacgcg tgctagcccg ggctcgagaa actgcaaaag gtcgagaaac tgcaaaaggt
      481 cgagaaactg caaaaggtcg agaaactgca aaaggtcgag aaactgcaaa aggtcgagaa
      541 actgcaaaag gtcgagaaac tgcaaaaggt cgagaaactg caaaaggtcg agaaactgca
      601 aaagagatct ggtaggcgtg tacggtggga ggtctatata agcagagctc g
//
