LOCUS       sequence-98914-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the RBD of human MSI2 in bacterial cells for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI2(8-193)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             664..681
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcatcatc atcatcatca cggcgaaaac
       61 ctgtattttc agggcggatc cggcacctcg ggcagcgcca acgactccca gcacgacccc
      121 ggtaaaatgt ttatcggtgg actgagctgg cagacctcac cagatagcct tagagactat
      181 tttagcaaat ttggagaaat tagagaatgt atggtcatga gagatcccac tacgaaacgc
      241 tccagaggct tcggtttcgt cacgttcgca gacccagcaa gtgtagataa agtattaggt
      301 cagccccacc atgagttaga ttccaagacg attgacccca aagttgcatt tcctcgtcga
      361 gcgcaaccca agatggtcac gagaacaaag aaaatatttg taggcgggtt atctgcgaac
      421 acagtagtgg aagatgtaaa gcaatatttc gagcagtttg gcaaggtgga agatgcaatg
      481 ctgatgtttg ataaaactac caacaggcac agagggtttg gctttgtcac ttttgagaat
      541 gaagatgttg tggagaaagt ctgtgagatt catttccatg aaatcaataa taaaatggta
      601 gaatgtaaga aagctcagcc gaaagaagtc atgttcccat gaaagcttgc ggccgcactc
      661 gagcaccacc accaccacca ctgagatccg gctgctaaca aag
//
