LOCUS       sequence-98915-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myc-tagged CYLD catalytic inactive (C601A) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: Myc-CYLD-C601A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..74
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     56..73
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             124..153
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             163..192
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             202..231
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             241..270
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             280..309
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             343..372
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcaatgctc gtttagggaa ccgccattct gcctggggac gtcggagcaa gcttgattta
       61 ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatcccatcg atttaaagct
      121 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct
      181 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      241 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      301 gaggacttga atgaaatgga gagcttgggc gacctcacca tggagcaaaa gctcatttct
      361 gaagaggact tgaattcagg ccggcccatg agttcaggct tatggagcca agaaaaagtc
      421 acttcaccct actgggaaga gcggattttt tacttgcttc ttcaagaatg cagcgttaca
      481 gacaaacaaa cacaaaagct ccttaaagta ccgaagggaa gtataggaca gtatattcaa
      541 gatcgttctg tggggcattc aaggattcct tctgcaaaag gcaagaaaaa tcagattgga
      601 ttaaaaattc tagagcaacc tcatgcagtt ctctttgttg atgaaaagga tgttgtagag
      661 ataaatgaaa agttcacaga gttacttttg gcaattacca attgtgagga gaggttcagc
      721 ctgtttaaaa acagaaacag actaagtaaa ggcctccaaa tagacgtggg ctgtcctgtg
      781 aaagtacagc tgagatctgg ggaagaaaaa tttcctggag ttgtacgctt cagaggaccc
      841 ctgttagcag agaggacagt ctccggaata ttctttggag ttgaattgct ggaagaaggt
      901 cgtgg
//
