LOCUS       sequence-98917-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eGFP reporter containing 8 copies of a gRNA binding site for 
            light-inducible dCas9 activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pGL3-Basic-8x-gRNA-eGFP
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..188)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     516..535
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcggccggc cgccccgact ctagactcga gcggccgcca ctgtgctgga tatctgcaga
       61 attccaccac actggactag tggatccgag ctcggtacca agcttaagtt taaacgctag
      121 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      181 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      241 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      301 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      361 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      421 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      481 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      541 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      601 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
      661 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      721 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg ttttac
//
