LOCUS       sequence-98921-e         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRedExpress2 in response to doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-Empty-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             99..152
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(333..353)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgag ctcggtaccc
       61 ggggatcctc tagtcagctg acgcgtttgg ttatgcatga gggcagagga agtcttctaa
      121 catgcggtga cgtggaggag aatcccggcc ctatggatag cactgagaac gtcatcaagc
      181 ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggcaag ccctacgagg gcacccagac cgccaagctg caggtgacca
      301 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
      361 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccaag
//
