LOCUS       sequence-98934-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-WT mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(150..203)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttggtcacct gcagcttggc ggtctgggtg ccctcgtagg gcttgccctc gccctcgccc
       61 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaag
      121 ggcttgatga cgttctcagt gctatccata gggccgggat tctcctccac gtcaccgcat
      181 gttagaagac ttcctctgcc ctcatgcata agcacctgat aaatcgcatt ggggtttgag
      241 cctgcaggac actgaggggc ggcgtcggga gacggggtct gggttccctg ttctgtgtcg
      301 cttagggatg ccccctctgg cggacccgga ggcgactctg gtggtgcatc tgccaaaagc
      361 agcgcaggcg cagcggggct gtctgtggag atgcgctcca ctatgctgga caggcagtcg
      421 aggctcgaca cagccgcact cttccctggc ctggactcgc gcaccgcctc actgtagtag
      481 gcggtgtcgt agccattctg ccgccggggg ccgcttgggg ggccgctgta atccatcatg
      541 ccatcagagc agttggagcg cgggctggac gcgtctgagt cgccactgta gtgctcgctg
      601 ccacggctct g
//
