LOCUS       sequence-98936-e         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP16 mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             126..359
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgagct
       61 cggtacccgg ggatcctcta gtcagctgac gcgccaccat gggccctaaa aagaagcgta
      121 aagtcgcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg
      181 tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg
      241 attccccggg tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg
      301 ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggg
      361 gaggcagtgg gggcggatca cgacgcgtca tggagcttct atcgccgcca ctccgggaca
      421 tagacttgac aggccccgac ggctctctct gctcctttga
//
