LOCUS       sequence-98937-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the RRM1 of mouse MSI1 in bacterial cells for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-103)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             665..682
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcatcat catcatcatc acggcgaaaa
       61 cctgtatttt cagggcggat cccagcccgg cctcgcctcc ccggactcgc cgcacgaccc
      121 ctgcaagatg ttcatcggag gactcagttg gcagaccacg caggaagggc tgcgcgaata
      181 cttcggccag ttcggggagg tgaaagagtg tctggtgatg cgggaccccc tgaccaaaag
      241 atccaggggt ttcggcttcg tcactttcat ggaccaggcg ggggtggata aagtgctggc
      301 gcaatcgcgg cacgagctcg actccaaaac aattgacccc aaggtggcct ttcctcgaag
      361 agcacagcct aagtaagtca ctcgaactaa gaagatcttc gtgggggggc tgtctgtgaa
      421 caccacggtg gaagatgtga aacactattt cgagcagttc ggaaaggtgg atgatgccat
      481 gctgatgttc gacaaaacca ccaacaggca cagagggttt ggatttgtca cgtttgagag
      541 cgaggacatc gtagagaaag tttgtgagat ccacttccat gaaatcaaca acaaaatggt
      601 ggaatgcaag aaagcccagc caaaggaggt gatgtccccg tgaaagcttg cggccgcact
      661 cgagcaccac caccaccacc actgagatcc ggctgctaac aaa
//
