LOCUS       sequence-98938-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CYLD (C601A) catalytic dead under control of Tet promoter with 
            N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: pTRE-Tight-FLAG-CYLD-C601A
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggggatcca tggattataa agatgacgac gataaaatga gttcaggctt atggagccaa
       61 gaaaaagtca cttcacccta ctgggaagag cggatttttt acttgcttct tcaagaatgc
      121 agcgttacag acaaacaaac acaaaagctc cttaaagtac cgaagggaag tataggacag
      181 tatattcaag atcgttctgt ggggcattca aggattcctt ctgcaaaagg caagaaaaat
      241 cagattggat taaaaattct agagcaacct catgcagttc tctttgttga tgaaaaggat
      301 gttgtagaga taaatgaaaa gttcacagag ttacttttgg caattaccaa ttgtgaggag
      361 aggttcagcc tgtttaaaaa cagaaacaga ctaagtaaag gcctccaaat agacgtgggc
      421 tgtcctgtga aagtacagct gagatctggg gaagaaaaat ttcctggagt tgtacgcttc
      481 agaggacccc tgttagcaga gaggacagtc tccggaatat tctttggagt tgaattgctg
      541 gaagaaggtc gtggtcaagg tttcactgac ggggtgtacc aagggaaaca gctttttcag
      601 tgtgatgaag attgtggcgt gtttgttgca ttggacaagc tagaactcat agaagatgat
      661 gacactgcat tggaaagtga ttacgcaggt cctggggaca caatgcaggt cgaacttcct
      721 cctttggaaa taaactccag agtttctttg aaggttggag aaacaataga atctggaaca
      781 gttatattct gtgatgtttt gccaggaaaa gaaagcttag gatattttgt tggtgtggac
      841 atggataacc ctattggcaa ctgggatgga agatttgatg g
//
