LOCUS       sequence-98946-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: GFP-CYLD-STOP
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(98..100,101..103,104..814)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(131..159)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     754..774
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    836..860
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agacaccatg gccagcaaag gagaagaact
      121 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      181 ttctgtcagt ggagagggtg aaggtgatgc tacatacgga aagcttaccc ttaaatttat
      241 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tctcttatgg
      301 tgttcaatgc ttttcccgtt atccggatca tatgaaacgg catgactttt tcaagagtgc
      361 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa
      421 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg
      481 tattgatttt aaagaagatg gaaacattct cggacacaaa ctcgagtaca actataactc
      541 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
      601 tcgccacaac attgaagatg gatccgttca actagcagac cattatcaac aaaatactcc
      661 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct
      721 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
      781 tgggattaca catggcatgg atgagctcta caaaagcggt tccggaccgg atcaaacaag
      841 tttgtacaaa aaagttggcg cgcccaccat gagttcaggc ttatggagcc aagaaaaagt
      901 cacttcaccc tactgggaag agcggatttt ttacttgctt cttcaagaat gcagcgttac
      961 agacaaacaa acacaaa
//
