LOCUS       sequence-98958-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for HR-mediated recombination to insert blasticidin 
            resistance and append a destabilization domain to the N-terminus of 
            TCOF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Park A, Won ST, Pentecost M, Bartkowski W, Lee B
  TITLE     CRISPR/Cas9 allows efficient and complete knock-in of a 
            destabilization domain-tagged essential protein in a human cell 
            line, allowing rapid knockdown of protein function.
  JOURNAL   PLoS One. 2014 Apr 17;9(4):e95101. doi: 
            10.1371/journal.pone.0095101. eCollection 2014.
  PUBMED    24743236
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-04-17"; volume: "9(4):e95101. doi"; pages: " 
            10.1371/journal.pone.0095101. eCollection 2014"
COMMENT     Sequence Label: BlastR-P2A-DD-TCOF1-donor
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc
       61 gccagtgtga tggatatctg cagaattcgc ccttttggcc ggcccttatt gcggtgctaa
      121 gcatatagtc acttttgtga ttctttgatt tgtctacctt actagagtgt aagctccagg
      181 agggctgaga gatggtgctc accattcgaa caccaggaag caccctgagt accggattaa
      241 cagttgaatt aatcaagtaa gaaatgactg taccacgagt tcgagtacca aagctgaccg
      301 tctcagtaca gacaatcgct aaaggcctga gttccagctc aactaagagg ctgtagggtc
      361 ccttttccag gcgctccccc gaggggctgg tcgcaatcca cccccgagac tcgcagcaac
      421 ctccttgccc cagggggggc cgcgcagagc ctagccacgt ggcgggagac ttaggaaact
      481 taaaataccc gctaccagga agggcgtgga ggtaagggaa gcgggaatta caaagttcgg
      541 cccctgcgag cacactttct ggcccaaccc actgcccgga tgaggaaagt gaggcccagg
      601 ggaactgaag tcgcagtggg aaggcagaga ttgcggctcc tccggtaccc ctggccgcgc
      661 cgtcgagcac tgcgggaccc ggctccgggc gcctgccggg tcgcctgggg acggggcgcg
      721 ggccgcgggc cgctgatctc cacatcttgc acgcggccct cgcacacggc ccacgaacgc
      781 ttacctggcc gctctgctcc ttcacttccc gcgccgcacg cacatagcca gcccgcagca
      841 gatggtggta gatcagggga agtagctccc gccgcttcct ggcctcggcc attgaaccgc
      901 cgccaccaga tccacctcca ccagatccac ctccaccttc cggttttaga agctccacat
      961 cgaagacga
//
