LOCUS       sequence-98959-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhance solubility and correct disulfide bond-formation of fused 
            protein expressed in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong 
            B, Kim SW, Choe H
  TITLE     Expression and purification of biologically active human FGF2 
            containing the b'a' domains of human PDI in Escherichia coli.
  JOURNAL   Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 
            10.1007/s12010-013-0140-3. Epub 2013 Mar 8.
  PUBMED    23471584
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12010-013-0140-3"; journalName: "Appl Biochem Biotechnol";
            date: "2013-05"; volume: "170"; issue: "1"; pages: "67-80"
COMMENT     Sequence Label: pDEST-hPDI
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..65
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgaa acaccatcac catcaccatc
       61 accatgagct cgacgcaccg gaagaagagg atcatgtcct ggttctgcgc aaaagcaact
      121 tcgcggaagc actggccgca cataaatatc tgctggtgga attttatgct ccttggtgcg
      181 gtcattgcaa agccctggcc ccggagtacg ccaaagccgc aggtaaactg aaagctgaag
      241 gtagcgaaat ccgcctggca aaggttgatg ctacggaaga gagtgacctg gcgcagcagt
      301 atggtgtccg cggctatcct acaattaaat tcttccgtaa cggtgatacc gcatctccaa
      361 aagaatatac cgctggtcgc gaggcggacg atattgttaa ctggctgaag aaacgcactg
      421 gccctgccgc aaccaccctg cctgatggcg ctgctgccga aagcctggtc gaaagtagcg
      481 aagttgccgt cattggtttc tttaaggatg tagaatctga cagtgccaaa cagtttctgc
      541 aagcggcaga ggctatcgat gacatcccgt tcggcatcac ctctaacagt gacgtattca
      601 gtaaatacca actggataaa gacggcgttg tgctgttcaa gaaatttgat gaaggtcgca
      661 acaactttga gggtgaggtg accaaggaga acctgctgga ttttattaag cacaaccaac
      721 tgccgctggt tattgaattt acagaacaaa cggc
//
