LOCUS       sequence-98961-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for HR-mediated recombination to insert blasticidin 
            resistance and append a destabilization domain to the N-terminus of 
            TCOF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Park A, Won ST, Pentecost M, Bartkowski W, Lee B
  TITLE     CRISPR/Cas9 allows efficient and complete knock-in of a 
            destabilization domain-tagged essential protein in a human cell 
            line, allowing rapid knockdown of protein function.
  JOURNAL   PLoS One. 2014 Apr 17;9(4):e95101. doi: 
            10.1371/journal.pone.0095101. eCollection 2014.
  PUBMED    24743236
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-04-17"; volume: "9(4):e95101. doi"; pages: " 
            10.1371/journal.pone.0095101. eCollection 2014"
COMMENT     Sequence Label: BlastR-P2A-DD-TCOF1-donor
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(178..234)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(244..639)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gcctcggatc acctcctgct tgcctagcat aaacttaaag ggcttgtttc tgtcccggga
       61 ggaatcgact ttctttccat cttcaagcat cccggtgtag tgcaccacac aggtctggcc
      121 gcgcttgggg aaggtgcgcc cgtctcctgg ggagatggtt tccacctgca ctcccatggg
      181 accggggttt tcttccacgt ctcctgcttg ctttaacaga gagaagttcg tggctccgga
      241 tccgccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
      301 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
      361 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
      421 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
      481 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
      541 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
      601 aatgagggtg gattcttctt gagacaaagg cttggccata cccgcgaccc ccggccggcc
      661 ggctacctgc acgccccgcc ttagtccccg cccgccactt ccctcgcgcc cttagacctc
      721 gcgcgcccca cttccggctc ctctttcgct ccggccgcgc ggcgcgccgg gaaacgtagt
      781 cccctctccg ggagtgg
//
