LOCUS       sequence-98962-e         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP16 mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(141..194)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgcagcttgg cggtctgggt gccctcgtag ggcttgccct cgccctcgcc ctcgatctcg
       61 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa gggcttgatg
      121 acgttctcag tgctatccat agggccggga ttctcctcca cgtcaccgca tgttagaaga
      181 cttcctctgc cctcatgcat aagcacctga taaatcgcat tggggtttga gcctgcagga
      241 cactgagggg cggcgtcggg agacggggtc tgggttccct gttctgtgtc gcttagggat
      301 gccccctctg gcggacccgg aggcgactct ggtggtgcat ctgccaaaag cagcgcaggc
      361 gcagcggggc tgtctgtgga gatgcgctcc actatgctgg acaggcagtc gaggctcgac
      421 acagccgcac tcttccctgg cctggactcg cgcaccgcct cactgtagta ggcggtgtcg
      481 tagccattct gccgccgggg gccgcttggg gggccgctgt aatccatcat gc
//
