LOCUS       sequence-98966-e         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 with single cysteine residue (M1C) for site-specific labelling
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, 
            Doudna JA
  TITLE     Programmable RNA recognition and cleavage by CRISPR/Cas9.
  JOURNAL   Nature. 2014 Sep 28. doi: 10.1038/nature13769.
  PUBMED    25274302
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Sep 28. doi: 10.1038/nature13769."
COMMENT     Sequence Label: 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S...
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acctccacgt tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt
       61 tagaagttgt ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc
      121 cgatttacca cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt
      181 aaggaaactt tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa
      241 ttcttggtcc acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt
      301 ttgcaattga gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat
      361 accttcttcg attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg
      421 attttcacgt gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac
      481 caattcatca acaactttta cagtctgta
//
