LOCUS       sequence-98967-e         369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a conditional luciferase cassette dependent on Cre 
            mediated recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-Floxed Luc
FEATURES             Location/Qualifiers
     source          1..369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..122
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(188..206)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
       61 tgaccaacta gtcatatgat aagaattcat aacttcgtat aatgtatgct atacgaagtt
      121 attagaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      181 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      241 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      301 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      361 atgcagtga
//
