LOCUS       sequence-98968-e         546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP64 mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             172..321
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             328..354
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt cgagctcggt
       61 acccggggat cctctagtca gctgacgcgt ttggttatgc atgccaccat gaaaactagt
      121 ggacaggccg gccaggctag cccgaaaaag aaacgcaaag ttgggcgcgc cgacgcgctg
      181 gacgatttcg atctcgacat gctgggttct gatgccctcg atgactttga cctggatatg
      241 ttgggaagcg acgcattgga tgactttgat ctggacatgc tcggctccga tgctctggac
      301 gatttcgatc tcgatatgtt aattaactac ccgtacgacg ttccggacta cgcttctgga
      361 ggcagtgggg gcggatcaat ggagcttcta tcgccgccac tccgggacat agacttgaca
      421 ggccccgacg gctctctctg ctcctttgag acagcagacg acttctatga tgatccgtgt
      481 ttcgactcac cagacctgcg cttttttgag gacctggacc cgcgcctggt gcacgtggga
      541 gccctc
//
