LOCUS       sequence-98969-e         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP64 mouse MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(156..209)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ccgcccttgg tcacctgcag cttggcggtc tgggtgccct cgtagggctt gccctcgccc
       61 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
      121 atgaagggct tgatgacgtt ctcagtgcta tccatagggc cgggattctc ctccacgtca
      181 ccgcatgtta gaagacttcc tctgccctca tgcataagca cctgataaat cgcattgggg
      241 tttgagcctg caggacactg aggggcggcg tcgggagacg gggtctgggt tccctgttct
      301 gtgtcgctta gggatgcccc ctctggcgga cccggaggcg actctggtgg tgcatctgcc
      361 aaaagcagcg caggcgcagc ggggctgtct gtggagatgc gctccactat gctggacagg
      421 cagtcgaggc tcgacacagc cgcactcttc cctggcctgg actcgcgcac cgcctcactg
      481 tagtaggcgg tgtcgtagcc attctgccgc cgggggccgc ttggggggcc gctgtaatcc
      541 atcatgccat cagagcagtt ggagcgcggg ctggacgcgt ctgagtcg
//
