LOCUS       sequence-98970-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for HR-mediated recombination to insert puromycin 
            resistance and append a destabilization domain to the N-terminus of 
            TCOF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Park A, Won ST, Pentecost M, Bartkowski W, Lee B
  TITLE     CRISPR/Cas9 allows efficient and complete knock-in of a 
            destabilization domain-tagged essential protein in a human cell 
            line, allowing rapid knockdown of protein function.
  JOURNAL   PLoS One. 2014 Apr 17;9(4):e95101. doi: 
            10.1371/journal.pone.0095101. eCollection 2014.
  PUBMED    24743236
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-04-17"; volume: "9(4):e95101. doi"; pages: " 
            10.1371/journal.pone.0095101. eCollection 2014"
COMMENT     Sequence Label: PuroR-P2A-DD-TCOF1-donor
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(793..812)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgaccatgat tacgccaagc ttggtaccga gctcggatcc cagcaatttc gggtgtcttt
       61 atgcctcagc ttagatatct ctctggggtt cggtgctcct ccttggggcg cccataggct
      121 gcatctgtcc ctgacaaggc acacaagata ttgaactata gttgcttggt tacggacagt
      181 cgtctctgtg agctttgagg gcaactgcca tgtattatgc atcttttgaa tccccctagc
      241 ctagccgggt gctagcccaa gttaggtgct tattacagat ttgttcagtt agcgaatgaa
      301 tcaatgaata aactggcaat tcgtgtctgg ttcatgggca ccaaaaaccg tgcgaaatgg
      361 ataaatgagc aggtaatggc agtacaaagt tcggatctag agaaagatcc agattaggga
      421 gttcagagat gagtaaaacg cagacccagc catcagagaa ttaacaagga agataaatct
      481 cttcaagaag ccagccggaa ggattaaaag agagtcgtcc aagtcccggc cggccccggg
      541 ggcgggtccc tgtctagccc cgcatgctcc gcgccaatgg gcggtattgt tgatcacgag
      601 tctccgcccc cttcgccttg aggggcgggg ccggccactc ccggagaggg gactacgttt
      661 cccggcgcgc cgcgcggccg gagcgaaaga ggagccggaa gtggggcgcg cgaggtctaa
      721 gggcgcgagg gaagtggcgg gcggggacta aggcggggcg tgcaggtagc cggccggccg
      781 ggggtcgcgg gtatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      841 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      901 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      961 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1021 accacgccgg a
//
