LOCUS       sequence-98973-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CibN-dCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-CibN-dCas9
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             145..651
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="NGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSSM
                     ITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCNE
                     KKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKEL
                     EKTDYI"
     CDS             658..681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatggact acaaagacca tgacggtgat
      121 tataaagatc atgacatcga tataaatgga gctataggag gtgacctttt gctcaatttt
      181 cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt
      241 gattctcctc tcgccggctt ctttgccgat tcttcaatga ttaccggcgg cgagatggac
      301 agctatcttt cgactgccgg tttgaatctt ccgatgatgt acggtgagac gacggtggaa
      361 ggtgattcaa gactctcaat ttcgccggaa acgacgcttg ggactggaaa tttcaagaaa
      421 cggaagtttg atacagagac taaggattgt aatgagaaga agaagaagat gacgatgaac
      481 agagatgacc tagtagaaga aggagaagaa gagaagtcga aaataacaga gcaaaacaat
      541 gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca agaaagaaga gaacaatttc
      601 tctaatgatt catctaaagt gacgaaggaa ttggagaaaa cggattatat tcatcacgat
      661 tacaaggatg acgatgacaa gatggg
//
